The membrane that separates the enamel organ and the dental papilla just prior to dentin formation is called:

 # The membrane that separates the enamel organ and the dental papilla just prior to dentin formation is called:

A. Inner enamel epithelium

B. Outer enamel epithelium

C. Membrana preformativa

D. Cell-free zone

The correct answer is C. Membrana preformativa.

The dental papilla is enclosed in the invaginated portion of the enamel organ. Before the inner enamel epithelium begins to produce enamel, the peripheral cells of the mesenchymal dental papilla differentiate into odontoblasts under the organizing influence of the epithelium. First, they assume a cuboidal form; later they assume a columnar form and acquire the specific potential to produce dentin. The dental papilla ultimately gives rise to dental pulp, once the dentin formation begins at the cuspal tip of the bell stage tooth germ. The basement membrane that separates the enamel organ and the dental papilla just prior to dentin formation is called the membrana preformativa.

Which of the following is true of cellular cementum?

 # Which of the following is true of cellular cementum?

A. Forms during root formation

B. Forms after eruption of the tooth

C. Seen at the coronal portion of the tooth

D. Formation is a slow process

The correct answer is B. Forms after eruption of the tooth. 

The cellular cementum is also known as secondary cementum as this is formed later than the AEFC (Acellular Extrinsic Fiber Cementum). The cellular cementum found in the apical third is mainly of two types—the cellular mixed fiber cementum which forms the bulk of secondary cementum and occupies the apical interradicular regions and the CIFC (Cellular intrinsic fiber cementum) which is present in the middle and apical third. These types are mainly involved in the adaptation and repair of cementum. Since the secondary cementum is formed rapidly the incremental lines are placed further apart than in AEFC (Acellular Extrinsic Fiber Cementum).

Reference: Orban's Oral Histology and  Embryology

Which of the following is indicative of ageing?

 # Which of the following is indicative of ageing?

A. Expression of dead tracts

B. Widening of predentin

C. Increase in tubular diameter

D. Increase in peritubular dentin

The correct answer is A. Expression of dead tracts. 

It is rather difficult to separate age and functional changes in dentin. Dentin is laid down throughout life. This dentin is termed secondary dentin. If dentin forms as a result of pathological process, like caries, it is termed tertiary dentin. Tertiary dentin shows irregularity in size, shape, number and arrangement of dentinal tubules. Dentinal tubules degenerate due to injury resulting in the formation of dead tracts. Mineralization of dentinal tubules results in the formation of sclerotic dentin. This makes the tooth to appear transparent in these areas.

Reference: Orban's Oral Histology and Embryology

The formation of hard tissue takes place in the:

 # The formation of hard tissue takes place in the:

A. Bud stage

B. Cap stage

C. Advanced bell stage

D. Early bell stage

The correct answer is C. Advanced bell stage.

Advanced bell stage is characterized by the commencement of mineralization and root formation. During the advanced bell stage, the boundary between inner enamel epithelium and odontoblasts outlines the future dentinoenamel junction. The formation of dentin occurs first as a layer along the future dentinoenamel junction in the region of future cusps and proceeds pulpally and apically.

Reference: Orban's Oral Histology and Embryology, 14th Edition

Paget’s test is done in the examination of

 # Paget’s test is done in the examination of:

A. A small swelling

B. Serum calcium

C. Urine analysis for alkaline phosphatase

D. Cytologic smear

The correct answer is A. A small swelling.

Paget's test is done for small swellings to know the consistency (cystic/solid). The centre and peripheries are palpated with index finger. Cystic swelling feels softer at centre than periphery whereas solid swelling feels firmer at center than periphery.

Which of the following is a general property of biofilms?

 # Which of the following is a general property of biofilms? 

a. Susceptible to host defenses. 

b. Limited habitat range. 

c. Open architecture.

d. Reduced virulence

The correct answer is C. Open architecture.

The close relationships between the architecture of a biofilm and its functional properties emphasizes the need to better describe and understand cell behavior, from single cell to multicellular scale, during biofilm structure development and maturation. Recent technological advances in methodologies including imaging and microscopy, molecular techniques, and physico-chemical assays, enabled the development of novel approaches dedicated to biofilm studies (Azeredo et al., 2017). The possibility to observe biofilm using high resolution and non-destructive methods now allows investigating the dynamics of multicellular structure development and the fate of each of its individual cellular components in parallel. For instance, the key architectural transitions and associated biophysical and genetic mechanisms supporting the developmental program of Vibrio cholerae biofilms have been recently disclosed using single-cell live imaging (Drescher et al., 2016; Yan et al., 2016). This kind of observations has clearly improved our understanding of spatio-temporal development of biofilms and has finally increasingly supported the intimate connection between structural modulations and the emergence of functional features and survival strategies. Indeed, the ability of biofilms to adapt their structure in response to internal or external stimuli, called hereafter the architectural plasticity, appears as a key factor affecting the fitness of individuals within the whole microbial community. Interestingly, the role of plasticity in bacterial survival was already demonstrated at the cellular scale. Bacteria are able to alter their morphology and to produce specific morphotypes conferring survival advantages in hostile environments. This was showed for numbers of bacterial pathogens for which filamentation is essential in the resistance to phagocytosis and overall for persistence during infection. 

Reference: https://www.frontiersin.org/articles/10.3389/fmicb.2017.01364/full

Boiling point of methyl methacrylate is:

# Boiling point of methyl methacrylate is:

A. Greater than B.P of water 

B. Lesser than B.P of water 

C. Equal to BP of water 

D. Is at normal room temperature 

The correct answer is A. Greater than Boiling Point of Water. 

Methyl methacrylate (MMA) is an organic compound with the formula CH2=C(CH3)COOCH3. This colorless liquid, the methyl ester of methacrylic acid (MAA), is a monomer produced on a large scale for the production of poly(methyl methacrylate) (PMMA).

Melting point : −48 °C (−54 °F; 225 K)

Boiling point : 101 °C (214 °F; 374 K)